LANCE Ultra cAMP assay offers unmatched sensitivity and S/B using a simple TR-FRET protocol. Ideal any Gs- or Gi-coupled GPCR assay, this Ultra-sensitive cAMP assay is supreme when working with low cell numbers, endogenous receptors, or difficult Gi-coupled receptors. Features: Unmatched assay sensitivity and signal stability-even with overnight incubation. Optimized screening of difficult targets and Gαi antagonists. Easier detection of cAMP response of endogenous receptors-use fewer cells per well. Robust sensitivity when miniaturized to 1536-well format uHTS. Trusted results: stable pharmacology over time with consistent rank order potencies. Reproducible results with highest values LANCE Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).

assay is based on competition between europium (Eu) chelate-labeled cAMP tracer and cellular cAMP binding sites on cAMP-specific monoclonal antibodies labeled with ULight dye. When antibodies are bound to Eu-labeled cAMP tracer, light pulse at 320 or 340 nm excites Eu chelate molecule of tracer. energy emitted by excited Eu chelate is transferred by FRET to ULight molecules on antibodies, which in turn emit light at 665 nm.

Revvity, we regard "impossibility" as inspiration and "inability" as the driving force. Revvity offers health science solutions, cutting - edge technologies and professional services, with its business covering the entire end - to - end process of scientific research exploration, development, diagnosis, and treatment. Relying on years of in - depth cultivation in fields such as translational multi - omics technology, biomarker identification, imaging, disease prediction, screening, detection and diagnosis, and informatics, Revvity is using the power of technology to break through the boundaries of human potential.

In 2023, Revvity's turnover exceeded $2.7 billion. It has more than 11,000 employees worldwide and provides diverse services for customers such as pharmaceutical and biotechnology enterprises, diagnostic laboratories, scientific research institutions and government agencies. The company is a member of the S&P 500 Index, and its customers are spread across more than 190 countries and regions around the world.