HTRF HUMAN IFN G KIT-Revvity
Order
Cat.NO Name Size
62HIFNGPEG HTRF HUMAN IFN G KIT  500 PTS
62HIFNGPEH HTRF HUMAN IFN G KIT  10K PTS

 

  • Product overview
  • Brand introduction
  • Q&A
  • Data download

IFN gamma is a pro-inflammatory cytokine secreted by T and NK cells in response to viral infection and or inflammatory conditions. IFN gamma plays a major role in innate and adaptive immunity by promoting T cell differentiation into TH1 and development of Treg, as well as by inhibiting T cell differentiation into TH17. Its anti-tumor properties have been used in treatment of osteoporosis and it is of great interest in immuno-oncology where it improves survival in patients with bladder or ovarian carcinoma.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

 

Product features

Specifications

 

Assay Points

500/10000

Assay Target Type

Kit

Assay Technology

HTRF

Brand

HTRF

Quantity

1

Shipping Conditions

Shipped in Dry Ice

Therapeutic Area

Oncology & Inflammation

Unit Size

500/10000 Assay Points

 

 

Assay principle

 

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

 

 

 

Assay data analysis

 

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

 

 

Assay details

Technical specifications of human IFN gamma kit

 

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent) 14 pg/mL &21 pg/mL
Range 21 – 4,000 pg/mL
Time to result Overnight at RT
Calibration NIBSC (82/587) value (IU/mL) = 0,019 x HTRF hIFN? value (pg/mL)
Species Human only

 

 

Analytical performance

Intra and inter assay

Intra-assay (n=24)


Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

 

Inter-assay (n=4)


Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

 

 

Dilutional linearity

​The excellent % of recovery obtained from these experiments show the good linearity of the assay.​

 

Spike and recovery

The same amount of recombinant cytokine was added to 2 different serum samples, and the set of responses obtained from a standard curve was compared to the calculated expected values. The ~ 100% of recovery observed validates the sample matrix used for this assay.

 

 

Assay validation

IFNg secretion in PBMCs stimulated with PMA and Ionomycin

PBMC plated at 50, 100, 200 and 400 kcells/well were stimulated for 3h with increasing concentrations of PMA (0, 1, 50 ng/mL) added to a 500 ng/mL Ionomycin solution. 16 µL of supernatants were then transferred into a white detection plate (384, low volume) to be analyzed with the Human IFN? Assay.

 

At Revvity, we regard "impossibility" as inspiration and "inability" as the driving force. Revvity offers health science solutions, cutting - edge technologies and professional services, with its business covering the entire end - to - end process of scientific research exploration, development, diagnosis, and treatment. Relying on years of in - depth cultivation in fields such as translational multi - omics technology, biomarker identification, imaging, disease prediction, screening, detection and diagnosis, and informatics, Revvity is using the power of technology to break through the boundaries of human potential.

 

In 2023, Revvity's turnover exceeded $2.7 billion. It has more than 11,000 employees worldwide and provides diverse services for customers such as pharmaceutical and biotechnology enterprises, diagnostic laboratories, scientific research institutions and government agencies. The company is a member of the S&P 500 Index, and its customers are spread across more than 190 countries and regions around the world.

video

全自动单细胞功能蛋白质组学分析系统产品介绍-IsoLight-IsoPlexis-艾飕普
×