HTRF IP-One Gq Detection Kit-Revvity
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Cat.NO Name Size
62IPAPEB HTRF IP-One Gq Detection Kit 1K PTS
62IPAPEC HTRF IP-One Gq Detection Kit 20K PTS
62IPAPEJ HTRF IP-One Gq Detection Kit 100K PTS

 

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HTRF IP-One assays detect the accumulation of inositol monophosphate, a stable downstream metabolite of IP3 induced by activation of a phospholipase C (PLC) cascade. This kit allows direct characterization of all types of compounds acting on Gq-coupled receptors in either adherent or suspension cells.

From high-throughput screening (HTS) to structure-activity relationships (SAR), IP-One has been shown to offer superior benefits over traditional assays

Product features

Specifications

Assay Points 1K/20K/500K
Assay Technology HTRF
Brand HTRF
Product Group Kit
Quantity 1
Shipping Conditions Shipped Ambient
Therapeutic Area

Cardiovascular

Infectious Diseases

Metabolism/Diabetes

NASH/Fibrosis

Neuroscience

Oncology & Inflammation

Rare Diseases

Unit Size 1K/20K/500K Assay Points

 

 

Assay principle

The kit is based on a competitive format involving a specific antibody labeled with cryptate (donor) and IP1 coupled to d2 (acceptor). Native IP1 produced by cells competes with d2-labeled IP1 for binding to monoclonal anti-IP1 Tb2+ cryptate. LiCl is added to the cell stimulation buffer, causing IP1 to accumulate upon receptor activation.

 

 

Assay protocol

The IP-One assay features a streamlined protocol with just two incubation steps: Cell stimulation by the ligand or target compounds IP1 detection using HTRF reagentsThis protocol requires only a single, one-hour incubation period following cell stimulation.

 

 

Assay details

Specificity of IP-One Gq assay

IP-One ensures signal specificity, measuring only IP-1, an analyte that is solely produced by the action of the phospholipase enzyme (PLC) and mediated by GPCRs. 

In contrast, calcium responses are complex in nature. Calcium release is frequently triggered by non-G protein mechanisms, including Ca2+ permeable channels, calcium pumps, calcium transporters.

 

Standard curve characteristics

Assay Window, sensitivity, reproducibility and specificity : IP-One makes no concession !

 

At Revvity, we regard "impossibility" as inspiration and "inability" as the driving force. Revvity offers health science solutions, cutting - edge technologies and professional services, with its business covering the entire end - to - end process of scientific research exploration, development, diagnosis, and treatment. Relying on years of in - depth cultivation in fields such as translational multi - omics technology, biomarker identification, imaging, disease prediction, screening, detection and diagnosis, and informatics, Revvity is using the power of technology to break through the boundaries of human potential.

 

In 2023, Revvity's turnover exceeded $2.7 billion. It has more than 11,000 employees worldwide and provides diverse services for customers such as pharmaceutical and biotechnology enterprises, diagnostic laboratories, scientific research institutions and government agencies. The company is a member of the S&P 500 Index, and its customers are spread across more than 190 countries and regions around the world.

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